بررسي اثر تجويز داخل سياهرگي دي-کلوپروستنول در زمان تلقيح مصنوعي بر ميزان آبستني گاو شيري در برنامهي Heatsynch

Transcription

1 بررسي اثر تجويز داخل سياهرگي دي-کلوپروستنول در زمان تلقيح مصنوعي بر ميزان آبستني گاو شيري در برنامهي Heatsynch ۳ ۲ *۱ محمد جواد بهزادي شهربابک ناصر شمس اسفندآبادي ابوالفضل شيرازي تقي تکتاز ۴ هفشجاني دريافت: پذيرش: چکيده am-pm. (P>0.05) Heatsynch F2α واژههاي کليدي: مقدمه 99 پست الکترونيک نويسندهي مسؤول: *

2 مواد و روش کار E F TMR(Total Mixed Ration) Heatsynch GnRH ampm ۱00

3 GLM (Cue-Mate, Bioniche Animal Health,Bellevile, NewZealand) FREQ CATMOD CTS-3300V, SIUI, Guangdong,China Chi-square P SAS نتايج Electrochemiluminescenceimmunoassay جدول ۱- P DIM DIM ۱0۱

4 ng/ml جدول ۲- b b a a a a Pfeifer بحث F E PG FSH LH F2α ۱0۲

5 PG F2α Archbald Gabriel منابع 1- Ahmad, N; Schrick, F.N; Butcher, R.L. and Inskeep, E.K; Effect of persistent follicles on early embryonic losses in beef cows. Biol Reprod; 1995; 52(5): Archbald, L.F; Tran, T; Massey, R. and Klapstein, E; Conception rates in dairy cows after timed-insemination and simultaneous treatment with gonadotrophin releasing hormone and/or prostaglandin F2 alpha. Theriogenology; 1992; 37(3): Bage, R; Gustafsson, H; Larsson, B; Forsberg, M. and Rodriguez-Martinez, H; Repeat breeding in dairy heifers: follicular dynamics and estrous cycle characteristics in relation to sexual hormone patterns. Theriogenology; 2002; 57(9): Çevik, M; Selcuk, M. and Doğan, S; Comparison of pregnancy rates after timed artificial insemination in ۱0۳

6 10- -Gatius, F; Yaniz, J.L; Santolaria, P; Murugavel, K; Guijarro, R; Calvo, E. and -Bejar, M; Reproductive performance of lactating dairy cows treated with cloprostenol atthe time of insemination. Theriogenology; 2004; 62(3-4): Gatius, F; -Bejar, M; Fenech, M. and Hunter, R.H; Ovulation failure and double ovulation in dairy cattle: risk factors and effects. Theriogenology; 2005; 63(5): Mihm, M; Curran, N; Hyttel, P; Knight, P.G; Boland, M.P. and Roche, J.F; Effect of dominant follicle persistence on follicular fluid oestradiol and inhibin and on oocyte maturation in heifers. J Reprod Fertil; 1999; 116(2): Morrison, D.G; Chandler, J.E; Chandler, L.S; Radintz, A.F. and Warner, M.B; Effect of prostaglandin F(2)alpha at insemination on sperm cell numbers and pregnancy rate in beef cattle. Theriogenology; 1988; 30(1): Nakao, T; Shirakawa, J; Tsurubayashi, M; Ohboshi, K; Abe, T; Sawamukai, Y; Saga, N; Tsunoda, N. and Kawata, K; A Ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas. Univ. Vet. Fak. Derg; 2010; 16(1): De Rensis, F. and Scaramuzzi, R.J; Heat stress and seasonal effects on reproduction in the dairy cow--a review. Theriogenology; 2003; 60(6): Dozier, B.L; Watanabe, K. and Duffy, D.M; Two pathways for prostaglandin F2 alpha synthesis by the primate periovulatory follicle. Reproduction; 2008; 136(1): Gabriel, H.G; Wallenhorst, S; Dietrich, E. and Holtz, W; The effect of prostaglandin F(2alpha) administration at the time of insemination on the pregnancy rate of dairy cows. Anim Reprod Sci.; 2011; 123(1-2): Gustafsson, H; Studies on follicular dynamics and hormonal asynchrony around ovulation as a potential cause of repeat breeding. Reprod. DomesT. Anim.; 1998; 33(3 4): Leonardi, C.E; Pfeifer, L.F; Rubin, M.I; Singh, J; Mapletoft, R.J; Pessoa, G.A; Bainy, A.M. and Silva, C.A; Prostaglandin F2alpha promotes ovulation in prepubertal heifers. Theriogenology; 2012; 78(7): ۱0۴

7 PGF2alpha as ovulatory stimulus for timed artificial insemination in cattle. Theriogenology; 2014 ; 81(5): Revah, I. and Butler, W.R; Prolonged dominance of follicles and reduced viability of bovine oocytes. J Reprod Fertil; 1996; 106(1): Sato, T; Jyujo, T; Iesaka, T; Ishikawa, J. and Igarashi, M; Follicle stimulating hormoneand prolactin release induced by prostaglandins in rat. Prostaglandins; 1974; 5(5): Sato, T; Taya, K; Jyujyo, T; Hirono, M. and Igarashi, M; The stimulatory effect of prostaglandins on luteinizing hormone release. Am. j. obstet. gyn.; 1974; 118(6): Stacey, N.E. and Pandey, S; Effects of indomethacin and prostaglandins on ovulation of goldfish. Prostaglandins; 1975; 9(4): Tsang, B.K; Ainsworth, L; Downey, B.R. and Armstrong, D.T; Preovulatory changes in cyclic AMP and prostaglandin concentrations in follicular fluid of gilts. Prostaglandins; 1979; 17(1): Turk, G; Gur, S; Sonmez, M; Bozkurt, T; Aksu, E.H. and Aksoy, H; Effect of exogenous GnRH at the time of artificial insemination on reproductive performance of Awassi ewes preliminary report on the treatment of ovulation failure in cows with gonadotropin-releasing hormone analog or human chorionic gonadotropin combined with insemination. Animal Reproduction Science; 1984; 7(6): Nasrollahi, S; Arab, M; Zamani, M. and Ghafeleh-bashi, M; Comparison between intravenous and intramuscular administration of prostaglandin E2 on management of missed abortion. J. res. health Sci.; 2008; 8(2): Neglia, G; Natale, A; Esposito, G; Salzillo, F; Adinolfi, L; Campanile, G; Francillo, M. and Zicarelli, L; Effect of prostaglandin F 2α at the time of AI on progesterone levels and pregnancy rate in synchronized Italian Mediterranean buffaloes. Theriogenology; 2008; 69(8): Orczyk, G.P. and Behrman, H.R; Ovulation blockade by aspirin or indomethacin--in vivo evidence for a role of prostaglandin in gonadotrophin secretion. Prostaglandins; 1972; 1(1(: Pfeifer, L.F; Leonardi, C.E; Castro, N.A; Viana, J.H; Siqueira, L.G; Castilho, E.M; Singh, J; Krusser, R.H. and Rubin, M.I; The use of ۱05

8 synchronized with progestagen- PMSG-PGF2alpha combination. Reprod Domest Anim; 2008; 43(3): Walsh, S; Williams, E. and Evans, A; A review of the causes of poor fertility in high milk producing dairy cows. Anim. Reprad. Sci.; 2011; 123(3): Wongkaweewit, K; Prommachart, P; Raksasub, R; Buranaamnuay, K; Techakumphu, M; De Rensis, F. and Tummaruk, P; Effect of the administration of GnRH or hcg on time of ovulation and the onset of estrus-to-ovulation interval in sows in Thailand. Trop Anim Health Prod; 2012; 44(3): ۱06

9 Vol.10 / No.2 Fall-Winter 2016/2017 Evaluation of the Effect of intravenous administration at the time of artificial insemination on conception rate of dairy cattle synchronized with heatsynchprotochol Behzadi-Shahrbabak, M.J. 1* ; Shams-Esfandabadi, N. 2 ; Shirazi, A. 3 ; Taktaz- Hafshejani, T Resident of Teriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord- Iran. 2. Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord- Iran. 3. Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord- Iran. 4. Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord branch, Islamic Azad University, Shahrekord- Iran. Recieved: 27 October 2015 Accepted: 14 February 2016 Summary Given to the proven role of endogenous on ovulation, the aim of the present study was to evaluate the effect of exogenous (D-cloprostenol) administration on conception rate in a large dairy herd. Total number of 362 lactating dairy cows in Zagrous Company of Shahrekord were selected for this study in summer (From 20 August to 6 September,2014). The estrus of all cows was synchronized by the Heatsynchprotochol and following heat detection the cows were artificially inseminated according to am-pm rule. The animals were submitted to two groups; the treatment group that received 150µg D-cloprostenol intravenously at the time of AI and the control group that didn t receive any more treatment at the time of AI. To measure the concentration of serum progesterone, blood samples were obtained from 13 cows of each group at the time of insemination and 4 and 15 days later. Pregnancy was diagnosed by rectal ultrasound examination on days after insemination. There was no significant difference between both groups in terms of conception rate and the serum progesterone levels (P>0.05). In conclusion, this study showed no beneficial effects of intravenous administration of D-cloprostenol on conception rate of dairy cows. Keywords: Ovulation failure, prostaglandin F2α, conception rate, progesterone, Heatsynch. * Corresponding Author dvm.behzadi@gmail.com 145 Iranian Journal of Vetrinary Clinical Sciences